Part:BBa_K786010:Experience

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Characterization of BBa_K786010

I. biobrick DNA length verification

In order to check the inserted biobrick DNA length, restriction digestion was performed by EcoRI and PstI, targeting on biobrick standard RFC10 site.

Applications of BBa_K786010

Together with, Negative Phototactic Construct for Blue Light Detection (BBa_K786001) Positive Phototactic Construct for Blue Light Detection (BBa_K786002), Phototactic Construct for Orange Light Detection (BBa_K786003) and the genome targeting CRISPR/Cas systems BBa_K786031. This part will have the following application.

Incorporation with BBa_K786031 as a new biosafety approach

Genetically-modified organisms have been put in use extensively throughout the last decade. Yet, concerns about such organisms causing undesirable effect to the environment once released have been raised. Our biobricks might be one of the answers to tackle such concerns. When the gene expression system for sensory rhodopsin is incorporated with the CRISPR/Cas system, bio-engineered cells would cleave their own DNA into nucleotides once exposed to natural light.
This system would be useful for bio-engineered cells that could be used to work in the dark. (e.g.: Biofuel synthesizing cells, human hormone-producing cells)

User Reviews

UNIQfa6e3da6880c9b08-partinfo-00000000-QINU UNIQfa6e3da6880c9b08-partinfo-00000001-QINU